New England harbor seal H3N8 influenza virus retains avian-like receptor specificity.

TitleNew England harbor seal H3N8 influenza virus retains avian-like receptor specificity.
Publication TypeJournal Article
Year of Publication2016
AuthorsHussein ITM, Krammer F, Ma E, Estrin M, Viswanathan K, Stebbins NW, Quinlan DS, Sasisekharan R, Runstadler J
JournalSci Rep
Date Published2016 Feb 18
KeywordsAnimals, Cell Line, Tumor, Chick Embryo, Dogs, Hemagglutinin Glycoproteins, Influenza Virus, Humans, Influenza A Virus, H3N8 Subtype, Madin Darby Canine Kidney Cells, Species Specificity, Viral Tropism, Virus Replication

<p>An influenza H3N8 virus, carrying mammalian adaptation mutations, was isolated from New England harbor seals in 2011. We sought to assess the risk of its human transmissibility using two complementary approaches. First, we tested the binding of recombinant hemagglutinin (HA) proteins of seal H3N8 and human-adapted H3N2 viruses to respiratory tissues of humans and ferrets. For human tissues, we observed strong tendency of the seal H3 to bind to lung alveoli, which was in direct contrast to the human-adapted H3 that bound mainly to the trachea. This staining pattern was also consistent in ferrets, the primary animal model for human influenza pathogenesis. Second, we compared the binding of the recombinant HAs to a library of 610 glycans. In contrast to the human H3, which bound almost exclusively to α-2,6 sialylated glycans, the seal H3 bound preferentially to α-2,3 sialylated glycans. Additionally, the seal H3N8 virus replicated in human lung carcinoma cells. Our data suggest that the seal H3N8 virus has retained its avian-like receptor binding specificity, but could potentially establish infection in human lungs. </p>

Alternate JournalSci Rep
PubMed ID26888262
PubMed Central IDPMC4757820
Grant ListGM98791 / GM / NIGMS NIH HHS / United States
T32 ES007020 / ES / NIEHS NIH HHS / United States
GM62116 / GM / NIGMS NIH HHS / United States
U54 GM062116 / GM / NIGMS NIH HHS / United States
HHSN266200700010C / / PHS HHS / United States
HHSN266200700010C / AI / NIAID NIH HHS / United States
R24 GM098791 / GM / NIGMS NIH HHS / United States